- Original article
- Open access
- Published:
Diagnostic value of 8-isoprostane and transforming growth factor-β in bronchial asthma patients
Egyptian Journal of Bronchology volume 12, pages 295–302 (2018)
Abstract
Background
Asthma is an airway inflammatory disease with functional and structural changes, leading to bronchial hyperreponsiveness and airflow obstruction. 8-Isoprostane (8-iso-PGF2α) is considered a marker of oxidative stress specific to lipid peroxidation, transforming growth factor β1 (TGFβ1) as an important fibrogenic and immunomodulatory factor known to induce structural changes associated with asthma.
Objective
We aimed to study the diagnostic value of 8-iso-PGF2α and TGFβ1 in asthmatic patients.
Patients and methods
Samples of serum and bronchoalveolar lavage fluid from 40 asthmatic patients (20 moderate and 20 severe) and 10 healthy volunteers were assessed for their levels of 8-iso-PGF2α and TGFβ1.
Results
Bronchoalveolar lavage 8-iso-PGF2α and TGFβ1 was higher in asthmatic patients. It was significantly increased with increased asthma severity.
Conclusion
Increased levels of TGFβ1 and 8-iso-PGF2α is associated with disease severity. However, there is need for continued exploration on the mechanisms responsible for these structural changes.
References
Bousquet J, Mantzouranis E, Cruz AA, Khaled N, Baena-Cagnani CE, Bleecker ER, et al. Uniform definition of asthma severity, control, and exacerbations:document presented for theWorld Health Organization Consultation on Severe Asthma. J Allergy Clin Immunol 2010;126:926–938.
Pilacik B, Nofer T, Wasowicz W. F2-Isoprostanes biomarkers of lipid peroxidation:their utility in evaluation of oxidative stress induced by toxic agents. Int J Occup Med Environ Health 2002;15:19–27.
Duvernelle C, Freund V, Frossard N. Transforming growth factor-beta and its role in asthma. Pulm Pharmacol Ther 2003;16:181–196.
Ghosh B, Sharma S, Nagpal K. Transforming growth factor beta1 gene (TGF β1) prediction of susceptibility for immunological disease 2010. US20107858763.
Howell JE, McAnulty RJ. TGF-beta:its role in asthma and therapeutic potential. Curr Drug Targets 2006;7:547–565.
Jingjing J, Gorge S. TGF-B2 reduces nitric oxide synthase mRNA through a ROCK-dependent pathway in airway epithelial cells. Curr Drug Targets 2011;7:547–565.
Mistry V, Doe C, Roach K, Morgan A, Wardlaw A, Pavord I, et al. Airway hyper responsiveness is dissociated from airway wall structural remodeling. J Allergy Clin Immunol 2008;122:335–341.
Moore W, Bleecker ER, Curran-Everett D, Erzurum SC, Ameredes BT, Bacharier L, et al. Characterization of the severe asthma phenotype by the National Heart, Lung and Blood Institute’s Severe Asthma Research Program. J Allergy Clin Immunol 2007;119:405–413.
Morrow JD, Roberts LJ. Isoprostanes:their role as an index of oxidant stress status in human pulmonary disease. Am J Respir Crit Care Med 2002;166:25–30.
Montuschi P, Corradi M, Ciabattoni G, Nightingale J, Kharitonov SA, Barnes PJ, et al. Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients. Am J Respir Crit Care Med 1999;160:216–220.
Wood LG, Fitzgerald DA, Gibson PG, Cooper DM, Garg ML. Lipid peroxidation as determined by plasma isoprostanes is related to disease severity in mild asthma. Lipids 2000;35:967–974.
Wood LC, Garg ML, Simpson JL, Mori TA, Croft KD, Wark PA, Gibson PG. Induced sputum 8-isoprostane concentrations in inflammatory airway diseases. Am J Respir Crit Care Med 2005;171:426–430.
Chang CH, Chang CH, Lui S. TGF1 is associated with asthma and modulates the severity of asthma. Resp Critical Care 2013;58:1343–1350.
Benayoun L, Druilhe A, Dombret MC, Aubier M, Pretolani M. Airway structural alterations selectively associated with sever asthma. Am J Respir Crit Care Med 2003;167:1360–1368.
Kharitonov SA, Barnes PJ. Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensation in asthma patients. Am J Respir Crit Care Med 1999;160:216–220.
Dworski R, Murray J, Roberts J, Oates J, Morrow J, Fisher L, et al. Allergen-induced synthesis of F2-isoprostanes in atopic asthmatics. Am J Respir Crit Care Med 1999;160:1947–1951.
Judith C, Mak W. Sustained elevation of systemic oxidative stress and inflammation in exacerbation and remission of asthma. Allergy 2013;29:561–581.
Samitasa K, Chorianopoulosb D, Vittorakisa S, Zervasa E, Economidoua E, Papatheodorouc G, et al. Exhaled cysteinyl-leukotrienes and 8-isoprostane in patients with asthma and their relation to clinical severity. Respir Med 2009;103:750–756.
Keskin O, Balaban S, Keskin M, Kucukosmanoglu E, Gogebakan B, Ozkars MY, et al. Relationship between exhalled leukottriene and 8 isoprostane level and asthma severity. Allergol Immunopathol (Madr) 2014;42:191–197.
Ozyilmaz E, Canbakan S, Capan N, Erturk A, Gulhan M. Correlation of plasma transforming growth factor beta1 with asthma control test. Asian Pac J Allergy Immunol 2008;26:185–189.
Manuyakorn W, Kamchaisatian W, Atamasirikul K, Sasisakulporn C, Direkwattanachai C, Benjaponpitak S. Serum TGF-beta1 in atopic asthma. Asian Pac J Allergy Immunol 2008;26:185–189.
Redington AE, Madden J, Frew AJ, Djukanovic R, Roche WR, Holgate ST, Howarth PH. Transforming growth factor-beta1 in asthma. Measurement in bronchoalveolar lavage fluid. Am J Respir Crit Care Med 1997;156:642–647.
Forno E, Lescher R, Strunk R, Weiss S, Fuhlbrigge A, Celedón JC. TGF-β1 expression in the asthmatics. J Allergy Clin Immunol 2011;127:741–749.
Joseph J, Benedict S, Badrinath P, Wassef S, Joseph M, Abdulkhalik S. Elevation of plasma transforming growth factor β1 levels in stable nonatopic asthma. Ann Allergy Asthma Immunol 2005;91:472–476.
Hong JG, Dong WF, Zhou XJ. Effect of montelukast sodium on TGF-β1 of peripheral blood mononuclear cells from children with mild persistent asthma. Eur J Cell Biol 2011;87:679–684.
Jarrett L, Chen JA, Figen J, Doğu L, Alişan N, Deniz E. Serum TGF-β, matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and tissue inhibitors of metalloproteinase (TIMP-1) levels in childhood asthma. Turk J Med 2008;38:415–419.
Magnon L, Fukushima T, Yamasaki A, Harada T. TGF-β1 expression in the asthmatic epithelium. Am J Respir Cell Mol Biol 2008;12:46–50.
Lommatzsch Xiang S, Qiu D. Changes of CD4+CD25+ regulatory T cells, IL-10 and TGF-β1 levels in peripheral blood in asthma. Am J Respir Crit Care Med 2009;179:356–362.
Ceyhan N, Ronmark J, Platts-Mills R. TGF-β1 slevels in induced sputum samples of asthmatic subjects. Ann Allergy Asthma Immunol 2008;10:146–148.
Author information
Authors and Affiliations
Corresponding author
Additional information
This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
Rights and permissions
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Frahat, A., Mansour, Y., Eldib, A. et al. Diagnostic value of 8-isoprostane and transforming growth factor-β in bronchial asthma patients. Egypt J Bronchol 12, 295–302 (2018). https://doi.org/10.4103/ejb.ejb_18_17
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.4103/ejb.ejb_18_17